Dual-glo luciferase assay system manual

The Dual-Glo® Luciferase Assay System is designed to allow high-throughput analysis of mammalian cells containing genes for firefly and Renilla luciferases, grown in or well plates. The Dual-Glo® Luciferase Reagent can be added directly to cells in growth medium without washing or preconditioning. The Nano-Glo ® Dual-Luciferase Reporter Assay System is designed to be used with many media types and has been validated for use with the following culture media containing 0–10% serum: DMEM, RPMI , McCoy’sAuthor: Promega Corporation. Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing dual reporter assays. In the DLR™ Reporter Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis, also known as sea pansy) luciferases are measured sequentially from a single sample.

The Dual-Luciferase® Reporter (DLR™) Assay System(a–c) provides an efficient means of performing dual-reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis, also known as sea pansy) luciferases are measured sequentially from a single sample. The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantitation of a stable luminescent signal from two reporter genes in a single sample. The convenient "add-mix-measure" protocol generates both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed. Luciferase Assay System(a,b) assays E Each system contains sufficient reagents for standard assays. Includes: • 1 vial Luciferase Assay Substrate (lyophilized) • 10ml Luciferase Assay Buffer • 30ml Luciferase Cell Culture Lysis Reagent, 5X • 1 Protocol Product Size Cat.# Luciferase Assay System Pack(a,b) 1, assays E

Dual-Glo™ Luciferase Assay System, for monitoring both firefly and Renilla luciferases expressed in mammalian cells in culture medium in and well. The Dual-Glo® Luciferase Assay System is designed to allow high-throughput analysis of mammalian cells containing genes for firefly and Renilla luciferases. 24 មករា Luciferase assays are widely used to quantify gene expression. Typically, they are used following cells transfection with an expression.